Foxtail Mosaic Virus-induced Flowering Assays in Monocot Crops

Yuan, C., Li, H., Qin, C., Zhang, X., Chen, Q., Zhang, P., Xu, X., He, M., Zhang, X., Tör, M. ORCID: https://orcid.org/0000-0002-4416-5048, Xue, D., Wang, H., Jackson, S., He, Y., Shi, N. and Hong, Yiguo (2020) Foxtail Mosaic Virus-induced Flowering Assays in Monocot Crops. Journal of Experimental Botany, 71 (10). pp. 3012-3023. ISSN Online ISSN 1460-2431 - Print ISSN 0022-0957

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Abstract

Virus-induced flowering (VIF) exploits RNA or DNA viruses to express flowering time genes to induce flowering in plants. Such plant virus-based tools have recently attracted widespread attention for their fundamental and applied uses in flowering physiology and in accelerating breeding in dicotyledonous crops and woody fruit-trees. We now extend this technology to a monocot grass and a cereal crop. Using the Foxtail mosaic virus-based VIF system, dubbed FoMViF, we showed that expression of florigenic Flowering Locus T (FT) genes can promote early flowering and spikelet development in proso millet, a C4 grass species with potential for nutritional food and biofuel resources, and in non-vermalized C3 wheat, a major food crop worldwide. Floral and spikelet/grain induction in the two monocot plants was caused by the virally expressed untagged or FLAG-tagged FT orthologues, and the florigenic activity of rice Hd3a was more pronounced than its dicotyledonous counterparts in proso millet. The FoMViF system is easy to perform and its efficacy to induce flowering and early spikelet/grain production is high. In addition to proso millet and wheat, we envisage that FoMViF will be also applicable to many economically important monocotyledonous food and biofuel crops.

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