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Multigene Editing Via CRISPR/Cas9 Guided By a Single-sgRNA Seed in Arabidopsis

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Yu, Z., Chen, Q., Chen, W., Zhang, X., Mei, F., Zhang, P., Zhao, M., Wang, X., Shi, N., Jackson, S. and Hong, Yiguo (2017) Multigene Editing Via CRISPR/Cas9 Guided By a Single-sgRNA Seed in Arabidopsis. Journal of Integrative Plant Biology. ISSN 1672-9072 Online: 1744-7909

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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/jipb.12...

Abstract

We report that a single-sgRNA seed is capable of guiding CRISPR/Cas9 to simultaneously edit multiple genes AtRPL10A, AtRPL10B and AtRPL10C in Arabidopsis. Our results also demonstrate that it is possible to use CRISPR/Cas9 technology to create AtRPL10 triple mutants which otherwise cannot be generated by conventional genetic crossing. Compared to other conventional multiplex CRISPR/Cas systems, a single sgRNA seed has the advantage of reducing off-target gene-editing. Such a single sgRNA seed-induced gene editing system might be also applicable to modify other homologous genes or even less-homologous sequences for multiple gene-editing in plants and other organisms. [Abstract copyright: This article is protected by copyright. All rights reserved.]

Item Type:	Article
Additional Information:	The full-text can be accessed via the Official URL.
Uncontrolled Discrete Keywords:	single-sgRNA, CRISPR/Cas9, AtRPL10A, AtRPL10B, AtRPL10C,
Subjects:	Q Science > Q Science (General)
Divisions:	College of Health, Life and Environmental Sciences > School of Science and the Environment
SWORD Depositor:	Prof. Pub Router
Depositing User:	Yiguo Hong
Date Deposited:	19 Jan 2018 10:19
Last Modified:	17 Jun 2020 17:20
URI:	https://worc-9.eprints-hosting.org/id/eprint/6266

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