Herbert, Rob, Orchard, C.B., Sorrell, D.A., Marchbank, A., Rogers, H.J., Suchomelova, P., Lipavská, H. and Azmi, A. (2005) Tobacco BY-2 Cells Expressing Fission Yeast cdc25 Bypass a G2/M Block on the Cell Cycle. The Plant Journal, 44 (2). pp. 290-299. ISSN 0960-7412
Full text not available from this repository. (Request a copy)Abstract
The mitotic inducer gene from Schizosaccharomyces pombe, Spcdc25, was used as a tool to investigate
regulation of G2/M in higher plants using the BY-2 (Nicotiana tabacum) cell line as a model. Spcdc25-
expressing BY-2 cells exhibited a reduced mitotic cell size through a shortening of the G2 phase. The cells often
formed isodiametric double files both in BY-2 cells and in cell suspensions derived from 35S::Spcdc25 tobacco
plants. In Spcdc25-expressing cells, the tobacco cyclin-dependent kinase, NtCDKB1, showed high activity in
early S phase, S/G2 and early M phase, whereas in empty vector cells CDKB1 activity was transiently high in
early S phase but thereafter remained lower. Spcdc25-expressing cells also bypassed a block on G2/M imposed
by the cytokinin biosynthetic inhibitor lovastatin (LVS). Surprisingly, cytokinins were at remarkably low levels
in Spcdc25-expressing cells compared with the empty vector, explaining why these cells retained mitotic
competence despite the presence of LVS. In conclusion, synchronised Spcdc25-expressing BY-2 cells divided
prematurely at a small cell size, and they exhibited premature, but sustained, CDKB1 activity even though
endogenous cytokinins were virtually undetectable.
Item Type: | Article |
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Additional Information: | The full-text cannot be supplied for this article. Please check availablity with your local library or Inter Library Requests Service. |
Uncontrolled Discrete Keywords: | Nicotiana tabacum, cell size, cytokinins, BY-2 cell line CDKA/B, plant cell cycle., SERG |
Subjects: | Q Science > QK Botany |
Divisions: | College of Health, Life and Environmental Sciences > School of Science and the Environment |
Depositing User: | Rob Herbert |
Date Deposited: | 27 Nov 2009 14:03 |
Last Modified: | 31 Jul 2020 14:18 |
URI: | https://worc-9.eprints-hosting.org/id/eprint/731 |
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