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ATR2Cala2 from Arabidopsis-infecting downy mildew requires 4 TIR-NLR immune receptors for full recognition

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Kim, D., Li, Y., Ahn, H., Woods-Tör, Alison, Cevik, V., Furzer, O., Ma, W., Tör, M. ORCID: https://orcid.org/0000-0002-4416-5048 and Jones, J. (2024) ATR2Cala2 from Arabidopsis-infecting downy mildew requires 4 TIR-NLR immune receptors for full recognition. New Phytologist. pp. 1-15. ISSN 0028-646X (print); 1469-8137 (web)

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Official URL: https://nph.onlinelibrary.wiley.com/doi/10.1111/np...

Abstract

Arabidopsis Col-0 RPP2A and RPP2B confer recognition of Arabidopsis downy mildew (Hyaloperonospora arabidopsidis [Hpa]) isolate Cala2, but the identity of the recognized ATR2Cala2 effector was unknown.

To reveal ATR2Cala2, an F2 population was generated from a cross between Hpa-Cala2 and Hpa-Noks1. We identified ATR2Cala2 as a non-canonical RxLR-type effector that carries a signal peptide, a dEER motif, and WY domains but no RxLR motif. Recognition of ATR2Cala2 and its effector function were verified by biolistic bombardment, ectopic expression and Hpa infection.

ATR2Cala2 is recognized in accession Col-0 but not in Ler-0 in which RPP2A and RPP2B are absent. In ATR2Emoy2 and ATR2Noks1 alleles, a frameshift results in an early stop codon. RPP2A and RPP2B are essential for the recognition of ATR2Cala2. Stable and transient expression of ATR2Cala2 under 35S promoter in Arabidopsis and Nicotiana benthamiana enhances disease susceptibility.

Two additional Col-0 TIR-NLR (TNL) genes (RPP2C and RPP2D) adjacent to RPP2A and RPP2B are quantitatively required for full resistance to Hpa-Cala2. We compared RPP2 haplotypes in multiple Arabidopsis accessions and showed that all four genes are present in all ATR2Cala2-recognizing accessions.

Item Type:	Article
Additional Information:	A pre-print version of this article was made available on bioRxiv prior to publication (https://doi.org/10.1101/2023.04.25.538220). The WRaP entry for this version is available here: https://eprints.worc.ac.uk/13093/
Uncontrolled Discrete Keywords:	Arabidopsis, plantimmunity, ATR2, Hyaloperonospora arabidopsidis, RPP2, RxLR effector, TNL
Divisions:	College of Health, Life and Environmental Sciences > School of Science and the Environment
Related URLs:	https://nph.onlinelibrary.wiley.com/jour... Publisher https://nph.onlinelibrary.wiley.com/
Copyright Info:	© 2024 The Authors; New Phytologist © 2024 New Phytologist Foundation, This is an open access article under the terms of theCreative Commons AttributionLicense, which permits use,distribution and reproduction in any medium, provided the original work is properly cited.
Depositing User:	Katherine Small
Date Deposited:	16 May 2024 09:17
Last Modified:	16 May 2024 09:25
URI:	https://worc-9.eprints-hosting.org/id/eprint/13923

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